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Fig. 4 | Microbial Cell Factories

Fig. 4

From: High-yield production of l-serine through a novel identified exporter combined with synthetic pathway in Corynebacterium glutamicum

Fig. 4

Verification of the function of NCgl0581. a The cell growth (squares) and l-serine titer (circles) of SSAAI (solid symbols) and NCgl0581 deletion strain SSAAIΔNCgl0581 (open symbols), respectively. b Plasmid pDXW-11-1 containing fragments of NCgl0581 (gray), intergenic region between NCgl0581 and NCgl0580 (black), and EGFP (green). c Plasmid pDXW-11-0 containing fragments of the intergenic region between NCgl0581 and NCgl0580 (black) and EGFP (green). d Fluorescence of the two strains, SSAAI ΔNCgl0581-1 (gray bar with slash) and SSAAIΔNCgl0581-0 (white bar). e Verification of NCgl0581 binding to the upstream region of SerE by using EMSA. Lane 1: the nuclear extracts with activated specific TF (positive control), Lane 2: the nuclear extracts without activated TF (negative control), Lane 3: Sample

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Microbial Cell Factories

ISSN: 1475-2859

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