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Fig. 2 | Microbial Cell Factories

Fig. 2

From: High-yield production of l-serine through a novel identified exporter combined with synthetic pathway in Corynebacterium glutamicum

Fig. 2

Fluorescence of cytoplasmic proteins and membrane proteins, and the result of amino acid export of SerE by using peptide feeding approach in SSAAI. a Fluorescence of cytoplasmic proteins and membrane proteins of SSAAI-10 (SSAAI harboring plasmid pDXW-10 only, gray bar with slash), SSAAI-egfp (SSAAI overexpressing EGFP protein with pDXW-10, gray bar), and SSAAI-serE-egfp (SSAAI overexpressing SerE-EGFP fusion protein with pDXW-10, white bar). b Extracellular concentration of l-serine in SSAAI (black circles) and serE deletion strain SSAAI ΔserE (gray circles) with 2 mM of the dipeptide Ser–Ser. Extracellular concentration of l-serine in SSAAI (white circles) without the dipeptide Ser–Ser. c Extracellular concentration of l-threonine in SSAAI (black circles) and serE deletion strain SSAAI ΔserE (gray circles) with 2 mM of the dipeptide Thr–Thr. Extracellular concentration of l-threonine in SSAAI (white circles) without the dipeptide Thr–Thr

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Microbial Cell Factories

ISSN: 1475-2859

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