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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Development of a whole-cell biocatalyst for diisobutyl phthalate degradation by functional display of a carboxylesterase on the surface of Escherichia coli

Fig. 2

Expression of recombinant fusion proteins: CarEW, CarEW-GFP and INPN-CarEW-GFP. (a) SDS-PAGE and (b) western blot of lysates of E. coli harboring pET-28a(+) series plasmids. Lane M, protein marker; Lane 1, E. coli cells harboring pET-28a(+); Lane 2, E. coli cells harboring pET-28a(+)/carEW; Lane 3, E. coli cells harboring pET-28a(+)/carEW/gfp; Lane 4, 5, 6, cytoplasmic fraction, inner membrane, and outer membrane of E. coli cells harboring pET-28a(+)/inpn/carEW/gfp, respectively. Anti-His monoclonal antibody was used as a 1:1000 dilution

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