Fig. 5

Immunization with MOMVs elicited cellular responses in spleen. One weeks after the final immunization, spleen tissues of chickens (n = 5) were sampled for the evaluation of immune gene expression. The qRT-PCR analysis was performed for the expression levels of cytokine genes: IFN-γ (a), IL-4 (b), IL-17 (c), IL-10 (d) and MHC-IIβ g (e). Data are representative of two independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; versus the control (PBS)