Table 3 Primers used in this work
Primer | Sequence (5′-3′) |
|---|---|
EGFP-F | ACGCGGATCCACCATGGGTAAAGGAGAAGAACTTTTCACTG |
IEGFP-F | ACGCGGATCCACCATGAAACTAAAGAATCAAGAT |
IEGFP-fuF | ATGAAGATTGTTTGAAAATGATGGGTAAAGGAGAAGAACT |
IEGFP-fuR | AGTTCTTCTCCTTTACCCATCATTTTCAAACAATCTTCAT |
I/EGFP-R | GACTCTCGAGTTTGTATAGTTCATCCATGCCA |
304I/EGFP-R | GAGCTCTTAGTGGTGGTGGTGGTGGTGCT |
Pac-F | GCCAAGCTTCAGGTAAATGGTTCTAACATGT |
Pac-R | GGTGGATCCCCTCCATCTCTTTTATTAAGAT |
Tac-F | GGATCCATCATCGAGCTCGAGGCAAACTCAGGTTTAAATATCGT |
Tac-R | GGTACCACTGCACAATTGTATTGAATGAT |
cry1Ia-F | ATAAAAGAGATGGAGGGATCCACCATGAAACTAAAGAATCAAGATAAG |
cry1IaD44-F | ATAAAAGAGATGGAGGGATCCACCATGTCTGAGTATGAAAATGTAGAG |
cry1IaD73-F | ATAAAAGAGATGGAGGGATCCACCATGCTAGGCGTTCCTTTTGCAGGACAAG |
cry1Ia-R | AACCTGAGTTTGCCTCGAGCTCCTACATGTTACGCTCAATATGGAGT |
Pi-F | CGCCAAGCTTGTATTTATAGGTGTTTGAAGT |
Pi-R | TGGTGGATCCCCCTCCACTTATACTATTATTTAAT |
pelBlaz-F | CTTAATAAAAGAGATGGAGGGGATCCACCATGAAATACCTGCTGCCGACC |
pelBlaz-R | GAAAAGTTCTTCTCCTTTACCCATGGTGGATCTGGCCATCGCCGGCTGGGCAGCGAGGA |
torAlaz-F | CTTAATAAAAGAGATGGAGGGGATCCACCATGAACAATAACGATCTCTTTCAG |
torAlaz-R | GAAAAGTTCTTCTCCTTTACCCATGGTGGATCTCGCTTGCGCCGCAGTCGCACGTCG |
egfpI-F | ATGGATGAACTATACAAACTCGAGCACCACCACCACCACCACATGAAACTAAAGAATCA |
egfpI-R | TATTTAAACCTGAGTTTGCCTCGAGCTCTTACATTTTCAAACAATCTTCATGAT |
mCherry-F | ACGCGGATCCACCATGGTGAGCAAGGGCGAGGAGGAT |
ImC-fuF | ATCATGAAGATTGTTTGAAAATGATGGTGAGCAAGGGCGAG |
ImC-fuR | CTCGCCCTTGCTCACCATCATTTTCAAACAATCTTCATGAT |
304I/mC-R | TGGTGAGCTCTTAGTGGTGGTGGTGGTGGTGCTCGAGCTTGTACAGCTCGTCCATG |
Hcat-F | CATCGGTCAACGGTACCA |
Hcat-R | TGGCACTGCTGACACTTC |
Hcat-Probe | FAM-AAGCCAATCATCACCAGCATCAGCCA-BHQ1 |
gfp-CF | GCCCTGTCCTTTTACCAGA |
gfp-CR | CATCCATGCCATGTGTAATCC |
gfp-Probe | FAM-CCATTACCTGTCCACACAATCTGCCCT-BHQ1 |