Plasmid | Characteristics | Source |
---|---|---|
pHT304 | Ampr, Ermr, Bacillus-E. coli shuttle vector | [64] |
p304ΔSacI | pHT304 without SacI recognition site | This work |
pAc-IeGFP | p304ΔSacI, Pac-Iegfp-Tac expression cassette | This work |
pAc-eGFP | p304ΔSacI, Pac-egfp-Tac expression cassette | This work |
pAc-pelBeGFP | p304ΔSacI, Pac-pelBegfp-Tac expression cassette | This work |
pAc-torAeGFP | p304ΔSacI, Pac-torAegfp-Tac expression cassette | This work |
pAc-eGFPI | p304ΔSacI, Pac-egfpI-Tac expression cassette | This work |
pI-IeGFP | p304ΔSacI, Pi-Iegfp-Tac expression cassette | This work |
pI-eGFP | p304ΔSacI, Pi-egfp-Tac expression cassette | This work |
pAc-ImCherry | p304ΔSacI, Pac-ImCherry-Tac expression cassette | This work |
pAc-mCherry | p304ΔSacI, Pac-mCherry-Tac expression cassette | This work |
pET28aDel | Kanr, pET28a without the sequence between RBS and BamHI | [61] |
pET28aDel2 | Kanr, add the GATATA at the 5′ of BamHI site of pET28aDel | This work |
p28aD-IeGFP | pET28aDel, PT7-Iegfp-TT7 expression cassette | This work |
p28aD-eGFP | pET28aDel, PT7-egfp-TT7 expression cassette | This work |
p22b-eGFP | pET22b, PT7-pelBegfp-TT7 expression cassette | This work |
pCMV-N-mCherry | mCherry gene | Beyotime biotechnology, China |
p28aD2-T-eGFP | pET28aDel2, PT7-Trx-egfp-TT7 expression cassette | This work |
p28aD2-N-eGFP | pET28aDel2, PT7-NusA-egfp-TT7 expression cassette | This work |
p28aD2-M-eGFP | pET28aDel2, PT7-MBP-egfp-TT7 expression cassette | This work |
p28aD2-MMP13 | pET28aDel2, PT7-mmp13-TT7 expression cassette | This work |
p28aD2-GDF8 | pET28aDel2, PT7-gdf8-TT7 expression cassette | This work |
p28aD2-I-MMP13 | pET28aDel2, PT7-I-mmp13-TT7 expression cassette | This work |
p28aD2-T-MMP13 | pET28aDel2, PT7-Trx-mmp13-TT7 expression cassette | This work |
p28aD2-I-GDF8 | pET28aDel2, PT7-I-gdf8-TT7 expression cassette | This work |
p28aD2-T-GDF8 | pET28aDel2, PT7-Trx-gdf8-TT7 expression cassette | This work |
p28aD-Cry1Ia | pET28aDel, PT7-cry1Ia-TT7 expression cassette | This work |
p28aD-Cry1IaD44 | pET28aDel, PT7-cry1IaD44-TT7 expression cassette | This work |
p28aD-Cry1IaD73 | pET28aDel, PT7- cry1IaD73-TT7 expression cassette | This work |
pAc-Cry1Ia | p304ΔSacI, Pac-cry1Ia-Tac expression cassette | This work |
pAc-Cry1IaD44 | p304ΔSacI, Pac-cry1IaD44-Tac expression cassette | This work |
pAc-Cry1IaD73 | p304ΔSacI, Pac-cry1IaD73-Tac expression cassette | This work |
Strain | Genotype | |
---|---|---|
E. coli | ||
TG1 | K12Δ(lac-pro), supE, thi, hsdD5, F– [traD36 proAB+lacIqlacZΔM15] | [65] |
BL21-star (DE3) | F–ompT hsdSB (r −B , m −B ) galdcmrne131 (DE3) | Invitrogen |
T304 | TG1 transformed with pHT304 | This work |
TAc-IeGFP | TG1 transformed with pAc-IeGFP | This work |
TAc-eGFP | TG1 transformed with pAc-eGFP | This work |
TAc-pelBeGFP | TG1 transformed with pAc-pelBeGFP | This work |
TAc-torAeGFP | TG1 transformed with pAc-torAeGFP | This work |
TAc-eGFPI | TG1 transformed with pAc-eGFPI | This work |
TAc-ImCherry | TG1 transformed with pAc-ImCherry | This work |
TAc-mCherry | TG1 transformed with pAc-mCherry | This work |
BL28aD | BL21-star (DE3) transformed with pET28aDel | This work |
BL28-IeGFP | BL21-star (DE3) transformed with p28aD-IeGFP | This work |
BL28-eGFP | BL21-star (DE3) transformed with p28aD-eGFP | This work |
BL22b | BL21-star (DE3) transformed with pET22b | This work |
BL22-eGFP | BL21-star (DE3) transformed with p22b-eGFP | This work |
BL28D2-T-eGFP | BL21-star (DE3) transformed with p28aD2-T-eGFP | This work |
BL28D2-N-eGFP | BL21-star (DE3) transformed with p28aD2-N-eGFP | This work |
BL28D2-M-eGFP | BL21-star (DE3) transformed with p28aD2-M-eGFP | This work |
BL28D2-MMP13 | BL21-star (DE3) transformed with p28aD2-MMP13 | This work |
BL28D2-GDF8 | BL21-star (DE3) transformed with p28aD2-GDF8 | This work |
BL28D2-I-MMP13 | BL21-star (DE3) transformed with p28aD2-I-MMP13 | This work |
BL28D2-T-MMP13 | BL21-star (DE3) transformed with p28aD2-T-MMP13 | This work |
BL28D2-I-GDF8 | BL21-star (DE3) transformed with p28aD2-I-GDF8 | This work |
BL28D2-T-GDF8 | BL21-star (DE3) transformed with p28aD2-T-GDF8 | This work |
BL28-Cry1Ia | BL21-star (DE3) transformed with p28aD-Cry1Ia | This work |
BL28-Cry1IaD44 | BL21-star (DE3) transformed with p28aD-Cry1Ia44 | This work |
BL28-Cry1IaD73 | BL21-star (DE3) transformed with p28aD-Cry1Ia73 | This work |
B. thuringiensis | ||
BMB171 | acrystalliferous mutant strain of YBT-1463 | [66] |
B304 | BMB171 transformed with pHT304 | This work |
BAc-IeGFP | BMB171 transformed with pAc-IeGFP | This work |
BAc-eGFP | BMB171 transformed with pAc-eGFP | This work |
BI-IeGFP | BMB171 transformed with pI-IeGFP | This work |
BI-eGFP | BMB171 transformed with pI-eGFP | This work |
BAc-ImCherry | BMB171 transformed with pAc-ImCherry | This work |
BAc-mCherry | BMB171 transformed with pAc-mCherry | This work |
BAc-Cry1Ia | BMB171 transformed with pAc-Cry1Ia | This work |
BAc-Cry1IaD44 | BMB171 transformed with pAc-Cry1IaD44 | This work |
BAc-Cry1IaD73 | BMB171 transformed with pAc-Cry1IaD73 | This work |