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Table 1 Plasmids and strains used and constructed in this work

From: The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host’s fluorescent intensity

Plasmid

Characteristics

Source

pHT304

Ampr, Ermr, Bacillus-E. coli shuttle vector

[64]

p304ΔSacI

pHT304 without SacI recognition site

This work

pAc-IeGFP

p304ΔSacI, Pac-Iegfp-Tac expression cassette

This work

pAc-eGFP

p304ΔSacI, Pac-egfp-Tac expression cassette

This work

pAc-pelBeGFP

p304ΔSacI, Pac-pelBegfp-Tac expression cassette

This work

pAc-torAeGFP

p304ΔSacI, Pac-torAegfp-Tac expression cassette

This work

pAc-eGFPI

p304ΔSacI, Pac-egfpI-Tac expression cassette

This work

pI-IeGFP

p304ΔSacI, Pi-Iegfp-Tac expression cassette

This work

pI-eGFP

p304ΔSacI, Pi-egfp-Tac expression cassette

This work

pAc-ImCherry

p304ΔSacI, Pac-ImCherry-Tac expression cassette

This work

pAc-mCherry

p304ΔSacI, Pac-mCherry-Tac expression cassette

This work

pET28aDel

Kanr, pET28a without the sequence between RBS and BamHI

[61]

pET28aDel2

Kanr, add the GATATA at the 5′ of BamHI site of pET28aDel

This work

p28aD-IeGFP

pET28aDel, PT7-Iegfp-TT7 expression cassette

This work

p28aD-eGFP

pET28aDel, PT7-egfp-TT7 expression cassette

This work

p22b-eGFP

pET22b, PT7-pelBegfp-TT7 expression cassette

This work

pCMV-N-mCherry

mCherry gene

Beyotime biotechnology, China

p28aD2-T-eGFP

pET28aDel2, PT7-Trx-egfp-TT7 expression cassette

This work

p28aD2-N-eGFP

pET28aDel2, PT7-NusA-egfp-TT7 expression cassette

This work

p28aD2-M-eGFP

pET28aDel2, PT7-MBP-egfp-TT7 expression cassette

This work

p28aD2-MMP13

pET28aDel2, PT7-mmp13-TT7 expression cassette

This work

p28aD2-GDF8

pET28aDel2, PT7-gdf8-TT7 expression cassette

This work

p28aD2-I-MMP13

pET28aDel2, PT7-I-mmp13-TT7 expression cassette

This work

p28aD2-T-MMP13

pET28aDel2, PT7-Trx-mmp13-TT7 expression cassette

This work

p28aD2-I-GDF8

pET28aDel2, PT7-I-gdf8-TT7 expression cassette

This work

p28aD2-T-GDF8

pET28aDel2, PT7-Trx-gdf8-TT7 expression cassette

This work

p28aD-Cry1Ia

pET28aDel, PT7-cry1Ia-TT7 expression cassette

This work

p28aD-Cry1IaD44

pET28aDel, PT7-cry1IaD44-TT7 expression cassette

This work

p28aD-Cry1IaD73

pET28aDel, PT7- cry1IaD73-TT7 expression cassette

This work

pAc-Cry1Ia

p304ΔSacI, Pac-cry1Ia-Tac expression cassette

This work

pAc-Cry1IaD44

p304ΔSacI, Pac-cry1IaD44-Tac expression cassette

This work

pAc-Cry1IaD73

p304ΔSacI, Pac-cry1IaD73-Tac expression cassette

This work

Strain

Genotype

 

E. coli

 TG1

K12Δ(lac-pro), supE, thi, hsdD5, F [traD36 proAB+lacIqlacZΔM15]

[65]

 BL21-star (DE3)

FompT hsdSB (r B , m B ) galdcmrne131 (DE3)

Invitrogen

 T304

TG1 transformed with pHT304

This work

 TAc-IeGFP

TG1 transformed with pAc-IeGFP

This work

 TAc-eGFP

TG1 transformed with pAc-eGFP

This work

 TAc-pelBeGFP

TG1 transformed with pAc-pelBeGFP

This work

 TAc-torAeGFP

TG1 transformed with pAc-torAeGFP

This work

 TAc-eGFPI

TG1 transformed with pAc-eGFPI

This work

 TAc-ImCherry

TG1 transformed with pAc-ImCherry

This work

 TAc-mCherry

TG1 transformed with pAc-mCherry

This work

 BL28aD

BL21-star (DE3) transformed with pET28aDel

This work

 BL28-IeGFP

BL21-star (DE3) transformed with p28aD-IeGFP

This work

 BL28-eGFP

BL21-star (DE3) transformed with p28aD-eGFP

This work

 BL22b

BL21-star (DE3) transformed with pET22b

This work

 BL22-eGFP

BL21-star (DE3) transformed with p22b-eGFP

This work

 BL28D2-T-eGFP

BL21-star (DE3) transformed with p28aD2-T-eGFP

This work

 BL28D2-N-eGFP

BL21-star (DE3) transformed with p28aD2-N-eGFP

This work

 BL28D2-M-eGFP

BL21-star (DE3) transformed with p28aD2-M-eGFP

This work

 BL28D2-MMP13

BL21-star (DE3) transformed with p28aD2-MMP13

This work

 BL28D2-GDF8

BL21-star (DE3) transformed with p28aD2-GDF8

This work

 BL28D2-I-MMP13

BL21-star (DE3) transformed with p28aD2-I-MMP13

This work

 BL28D2-T-MMP13

BL21-star (DE3) transformed with p28aD2-T-MMP13

This work

 BL28D2-I-GDF8

BL21-star (DE3) transformed with p28aD2-I-GDF8

This work

 BL28D2-T-GDF8

BL21-star (DE3) transformed with p28aD2-T-GDF8

This work

 BL28-Cry1Ia

BL21-star (DE3) transformed with p28aD-Cry1Ia

This work

 BL28-Cry1IaD44

BL21-star (DE3) transformed with p28aD-Cry1Ia44

This work

 BL28-Cry1IaD73

BL21-star (DE3) transformed with p28aD-Cry1Ia73

This work

B. thuringiensis

 BMB171

acrystalliferous mutant strain of YBT-1463

[66]

 B304

BMB171 transformed with pHT304

This work

 BAc-IeGFP

BMB171 transformed with pAc-IeGFP

This work

 BAc-eGFP

BMB171 transformed with pAc-eGFP

This work

 BI-IeGFP

BMB171 transformed with pI-IeGFP

This work

 BI-eGFP

BMB171 transformed with pI-eGFP

This work

 BAc-ImCherry

BMB171 transformed with pAc-ImCherry

This work

 BAc-mCherry

BMB171 transformed with pAc-mCherry

This work

 BAc-Cry1Ia

BMB171 transformed with pAc-Cry1Ia

This work

 BAc-Cry1IaD44

BMB171 transformed with pAc-Cry1IaD44

This work

 BAc-Cry1IaD73

BMB171 transformed with pAc-Cry1IaD73

This work