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Table 1 Plasmids and strains used and constructed in this work

From: The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host’s fluorescent intensity

Plasmid Characteristics Source
pHT304 Ampr, Ermr, Bacillus-E. coli shuttle vector [64]
p304ΔSacI pHT304 without SacI recognition site This work
pAc-IeGFP p304ΔSacI, Pac-Iegfp-Tac expression cassette This work
pAc-eGFP p304ΔSacI, Pac-egfp-Tac expression cassette This work
pAc-pelBeGFP p304ΔSacI, Pac-pelBegfp-Tac expression cassette This work
pAc-torAeGFP p304ΔSacI, Pac-torAegfp-Tac expression cassette This work
pAc-eGFPI p304ΔSacI, Pac-egfpI-Tac expression cassette This work
pI-IeGFP p304ΔSacI, Pi-Iegfp-Tac expression cassette This work
pI-eGFP p304ΔSacI, Pi-egfp-Tac expression cassette This work
pAc-ImCherry p304ΔSacI, Pac-ImCherry-Tac expression cassette This work
pAc-mCherry p304ΔSacI, Pac-mCherry-Tac expression cassette This work
pET28aDel Kanr, pET28a without the sequence between RBS and BamHI [61]
pET28aDel2 Kanr, add the GATATA at the 5′ of BamHI site of pET28aDel This work
p28aD-IeGFP pET28aDel, PT7-Iegfp-TT7 expression cassette This work
p28aD-eGFP pET28aDel, PT7-egfp-TT7 expression cassette This work
p22b-eGFP pET22b, PT7-pelBegfp-TT7 expression cassette This work
pCMV-N-mCherry mCherry gene Beyotime biotechnology, China
p28aD2-T-eGFP pET28aDel2, PT7-Trx-egfp-TT7 expression cassette This work
p28aD2-N-eGFP pET28aDel2, PT7-NusA-egfp-TT7 expression cassette This work
p28aD2-M-eGFP pET28aDel2, PT7-MBP-egfp-TT7 expression cassette This work
p28aD2-MMP13 pET28aDel2, PT7-mmp13-TT7 expression cassette This work
p28aD2-GDF8 pET28aDel2, PT7-gdf8-TT7 expression cassette This work
p28aD2-I-MMP13 pET28aDel2, PT7-I-mmp13-TT7 expression cassette This work
p28aD2-T-MMP13 pET28aDel2, PT7-Trx-mmp13-TT7 expression cassette This work
p28aD2-I-GDF8 pET28aDel2, PT7-I-gdf8-TT7 expression cassette This work
p28aD2-T-GDF8 pET28aDel2, PT7-Trx-gdf8-TT7 expression cassette This work
p28aD-Cry1Ia pET28aDel, PT7-cry1Ia-TT7 expression cassette This work
p28aD-Cry1IaD44 pET28aDel, PT7-cry1IaD44-TT7 expression cassette This work
p28aD-Cry1IaD73 pET28aDel, PT7- cry1IaD73-TT7 expression cassette This work
pAc-Cry1Ia p304ΔSacI, Pac-cry1Ia-Tac expression cassette This work
pAc-Cry1IaD44 p304ΔSacI, Pac-cry1IaD44-Tac expression cassette This work
pAc-Cry1IaD73 p304ΔSacI, Pac-cry1IaD73-Tac expression cassette This work
Strain Genotype  
E. coli
 TG1 K12Δ(lac-pro), supE, thi, hsdD5, F [traD36 proAB+lacIqlacZΔM15] [65]
 BL21-star (DE3) FompT hsdSB (r B , m B ) galdcmrne131 (DE3) Invitrogen
 T304 TG1 transformed with pHT304 This work
 TAc-IeGFP TG1 transformed with pAc-IeGFP This work
 TAc-eGFP TG1 transformed with pAc-eGFP This work
 TAc-pelBeGFP TG1 transformed with pAc-pelBeGFP This work
 TAc-torAeGFP TG1 transformed with pAc-torAeGFP This work
 TAc-eGFPI TG1 transformed with pAc-eGFPI This work
 TAc-ImCherry TG1 transformed with pAc-ImCherry This work
 TAc-mCherry TG1 transformed with pAc-mCherry This work
 BL28aD BL21-star (DE3) transformed with pET28aDel This work
 BL28-IeGFP BL21-star (DE3) transformed with p28aD-IeGFP This work
 BL28-eGFP BL21-star (DE3) transformed with p28aD-eGFP This work
 BL22b BL21-star (DE3) transformed with pET22b This work
 BL22-eGFP BL21-star (DE3) transformed with p22b-eGFP This work
 BL28D2-T-eGFP BL21-star (DE3) transformed with p28aD2-T-eGFP This work
 BL28D2-N-eGFP BL21-star (DE3) transformed with p28aD2-N-eGFP This work
 BL28D2-M-eGFP BL21-star (DE3) transformed with p28aD2-M-eGFP This work
 BL28D2-MMP13 BL21-star (DE3) transformed with p28aD2-MMP13 This work
 BL28D2-GDF8 BL21-star (DE3) transformed with p28aD2-GDF8 This work
 BL28D2-I-MMP13 BL21-star (DE3) transformed with p28aD2-I-MMP13 This work
 BL28D2-T-MMP13 BL21-star (DE3) transformed with p28aD2-T-MMP13 This work
 BL28D2-I-GDF8 BL21-star (DE3) transformed with p28aD2-I-GDF8 This work
 BL28D2-T-GDF8 BL21-star (DE3) transformed with p28aD2-T-GDF8 This work
 BL28-Cry1Ia BL21-star (DE3) transformed with p28aD-Cry1Ia This work
 BL28-Cry1IaD44 BL21-star (DE3) transformed with p28aD-Cry1Ia44 This work
 BL28-Cry1IaD73 BL21-star (DE3) transformed with p28aD-Cry1Ia73 This work
B. thuringiensis
 BMB171 acrystalliferous mutant strain of YBT-1463 [66]
 B304 BMB171 transformed with pHT304 This work
 BAc-IeGFP BMB171 transformed with pAc-IeGFP This work
 BAc-eGFP BMB171 transformed with pAc-eGFP This work
 BI-IeGFP BMB171 transformed with pI-IeGFP This work
 BI-eGFP BMB171 transformed with pI-eGFP This work
 BAc-ImCherry BMB171 transformed with pAc-ImCherry This work
 BAc-mCherry BMB171 transformed with pAc-mCherry This work
 BAc-Cry1Ia BMB171 transformed with pAc-Cry1Ia This work
 BAc-Cry1IaD44 BMB171 transformed with pAc-Cry1IaD44 This work
 BAc-Cry1IaD73 BMB171 transformed with pAc-Cry1IaD73 This work