Skip to main content
Fig. 9 | Microbial Cell Factories

Fig. 9

From: The signal peptide of Cry1Ia can improve the expression of eGFP or mCherry in Escherichia coli and Bacillus thuringiensis and enhance the host’s fluorescent intensity

Fig. 9

Expression analysis of mCherry and ImCherry in E. coli TG1 and Bt BMB171 strain. a The fluorescent intensities of the harvested TG1 cells expressing mCherry or ImCherry were monitored at different times (4, 8, 10, 12 and 24 h after inoculation). The slit widths of EX/EM were 1.5 nm and 3 nm, and the detections were conducted in high sensitivity. The fluorescent signals of B304 cells cannot be detected. The error bars indicate standard error of mean. The significant differences of the fluorescent intensities between TAc-ImCherry and TAc-mCherry cells at corresponding time were indicated by single asterisk (p < 0.05) or double asterisks (p < 0.01). b Comparison of the collected E. coli TG1 cells when they were incubated for 24 h. c Western blot analysis of the expression of mCherry and ImCherry in E. coli TG1 strain. “-” lane is negative control which prepared from T304 cells sampled at 12 h after inoculation. TAcImCherry and TAcmCherry cells were separately taken at 4, 8, 10, 12 and 24 h after inoculation. “M” represents the molecular weight standards. d, e The fluorescent intensities of the supernatants of cell cultures (panel D, slit widths of EX/EM = 3/5 nm in high sensitivity) or the harvested Bt cells (e slit widths of EX/EM = 3/3 nm in high sensitivity) at corresponding culture time. The fluorescent signals of B304 cells cannot be detected. The error bars indicate standard error of mean. The significant differences of the fluorescent intensities between BAc-ImCherry and BAc-mCherry cells at corresponding time were indicated by double asterisks (p < 0.01). f Western blot analysis of the expression of ImCherry in Bt BMB171 strain. “S” represents the supernatant of cell culture and the “P” means the resuspended cells by PBS buffer. “M” represents the molecular weight standards

Back to article page