Fig. 8
Fluorescent intensities analysis of the Pi promoter regulated eGFP and IeGFP in Bt strain. The fluorescent intensities of the resuspended cells by PBS buffer (a) and the supernatants of cell cultures (b) were detected respectively. The samples were obtained at different times (9, 12, 24, 36, 48, 60 and 72 h after inoculation). The slit widths of EX/EM were 3/3 nm and the detections were conducted in high sensitivity. The fluorescent signals of B304 cells cannot be detected and the fluorescent intensity of the 36 h cells of BI-IeGFP strain was beyond the limit (1000 A.U.). The significant difference of the fluorescent intensities between BI-IeGFP and BI-eGFP strains at corresponding time were indicated by single asterisk (p < 0.05). The error bars indicate standard error of mean