Fig. 6
Expression analysis of the different fluorescent proteins in E. coli strain. a The expression of eGFP (lane “e”), IeGFP (lane “Ie”), eGFP-I (lane “eI”), pelB-eGFP (lane “pe”) and torA-eGFP (lane “te”) at different times (4, 8, 10, 12 and 24 h after inoculation) were analyzed by western blot. Lane “-” is the negative control which prepared from T304 cells sampled at the corresponding times respectively. Lane “M” is the molecular weight standards. The arrows indicate the expressed IeGFP at different times. b The optical densities at 600 nm (OD600) of the E. coli strains expressing the different fluorescent proteins were monitored during the first 6 h after inoculation. The error bars indicate standard deviation of mean. c The fluorescent intensities of the samples obtained at corresponding times in panel a (slit widths of EX/EM = 3/5 nm in low sensitivity). The fluorescent signals of T304 cells cannot be detected. The error bars indicate standard error of mean