Fig. 4

The expression analysis of IeGFP in E. coli BL21-star (DE3). IeGFP (lane “I”) and eGFP (lane “e”) expressed in E. coli BL21-star (DE3) at the different induction temperatures were analyzed by SDS-PAGE. Lane “-” is the negative controls sampled from the BL28aD cell culture and lane “M” is the molecular weight standards. The arrow indicates the IeGFP expressed at 16 °C