Skip to main content
Fig. 4 | Microbial Cell Factories

Fig. 4

From: Enhanced production of recombinant proteins in Corynebacterium glutamicum by constructing a bicistronic gene expression system

Fig. 4

Protein expression levels of the top three strongest BCD expression vectors. pXMJ19-ALDH, pXMJ19-ADH, pXMJ19-RamA, pXMJ19-BoIFN-α, pXMJ19-gD, pXMJ19-PΙNP were used as control, pXMJ19-0 were used as CK. Arrow represents the target protein. Lane M: Protein Marker; lanes 1–3 represented expression vectors: pbtac-HT-8, pbtac-HT-11and pbtac-HP-9; lane 4: control, protein expression with a monocistronic Ptac promoter; lane 5: CK, pXMJ19 without exogenous protein genes. a SDS-PAGE and western blotting analysis of ALDH. b SDS-PAGE and western blot analysis of ADH. c SDS-PAGE and western blotting analysis of RamA. d SDS-PAGE and western blotting analysis of BoIFN-α. e SDS-PAGE and western blotting analysis of gD. f SDS-PAGE and western blotting analysis of PΙNP

Back to article page