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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enhanced production of recombinant proteins in Corynebacterium glutamicum by constructing a bicistronic gene expression system

Fig. 3

The expression stability and translation efficiency of the top three strongest enhanced expression vectors. Fluorescence intensity was normalized to OD600 of each construct. The asterisks indicate a significant difference between BCD expression vector and the original vector containing a Ptac at *P < 0.05 and **P < 0.01 using the t-test. a Fluorescence intensity of pbtac-HT-8, pbtac-HT-11 and pbtac-HP-9 under different culture conditions. b The EGFP background expression level and induction efficiency of the top three strongest vectors (*P < 0.05, **P < 0.01). c Relative transcriptional level and relative translation efficiency of EGFP for each enhanced expression vector (*P < 0.05, **P < 0.01)

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