Table 2 Strains and plasmids used in this study
From: Metabolic engineering of Escherichia coli for efficient production of l-alanyl-l-glutamine
Strains/plasmids | Characteristics | Source |
|---|---|---|
Strains | ||
E. coli BW25113 | IacIqrrnBT14∆lacZWJ16hsdR514∆araBADAH33∆rhaBADLD78 | Invitrogen |
AQ02 | E. coli BW25113, ∆glnE, ∆glnB | This study |
AQ04 | E. coli BW25113, ∆glsA, ∆glsB | This study |
AQ06 | E. coli BW25113, ∆glnE, ∆glnB, ∆glsA, ∆glsB, ∆lpxM | This study |
AQ09 | E. coli BW25113, ∆pepA, ∆ pepB, ∆ pepD, ∆ pepN, ∆dpp | This study |
AQ10 | E. coli BW25113, ∆glnE, ∆glnB, ∆glsA, ∆glsB, ∆lpxM, ∆pepA, ∆ pepB, ∆ pepD, ∆ pepN, ∆dpp | This study |
p11/AQ10 | AQ10 expressing p11 | This study |
p12/AQ10 | AQ10 expressing p12 | This study |
p13/AQ10 | AQ10 expressing p13 | This study |
p14/AQ10 | AQ10 expressing p14 | This study |
p15/AQ10 | AQ10 expressing p15 | This study |
p16/AQ10 | AQ10 expressing p16 | This study |
Plasmids | ||
pYB1a | P15A origin, pBAD promoter, AmpR | Our lab |
pYB1s | P15A origin, pBAD promoter, StrR | Our lab |
p00 | glnA from Corynebacterium glutamicum cloned into pYB1a | This study |
p01 | bacD from Bacillus subtilis cloned into pYB1a | This study |
p02 | bacD from Bacillus altitudinis cloned into pYB1a | This study |
p03 | bacD from Beta vulgaris cloned into pYB1a | This study |
p04 | bacD from Vibrio campbellii cloned into pYB1a | This study |
p05 | bacD from Streptomyces rubrolavendulae cloned into pYB1a | This study |
p06 | bacD from Bacillus safensis cloned into pYB1a | This study |
p07 | bacD from Bifidobacterium longum subsp. Infantis cloned into pYB1a | This study |
p08 | bacD from Brevibacillus laterosporus cloned into pYB1a | This study |
p09 | bacD from Perkinsus marinus cloned into pYB1a | This study |
p10 | bacD from Pseudomonas fluorescens cloned into pYB1a | This study |
p11 | CgglnA-BsbacD cloned into pYB1s | This study |
p12 | BsbacD-CgglnA cloned into pYB1s | This study |
p13 | CgglnA-BabacD cloned into pYB1s | This study |
p14 | BabacD-CgglnA cloned into pYB1s | This study |
p15 | BsbacD mRNA corresponding translation initiation rate was predicted to be 176300 | This study |
p16 | BsbacD mRNA corresponding translation initiation rate was predicted to be 295800 | This study |