Table 1 Production of AQ using engineered E. coli
From: Metabolic engineering of Escherichia coli for efficient production of l-alanyl-l-glutamine
Strain | Deletion of gene | Overexpression of genes | Process | AQ | References |
|---|---|---|---|---|---|
E. coli JKYPQ3/pPE167 | ΔpepAΔpepBΔpepDΔpepNΔdppΔproΔglnBΔglnEΔputA | l-amino acid α-ligase (Lal/BacD) and l-alanine dehydrogenase (Ald) | Fermentation from glucose | 7.4 mM in test tube in 47 h 100 mM (24.7 g/L) in fermenter in 47 h | [20] |
E. brevis ATCC 14234 | None | α-amino acid ester acyltransferase | Enzymatic production by purified enzyme from AlaOMe and Gln | 83 mM (18.0 g/L) in 1 h | [31] |
E. coli SP1/pSaet | ΔpepD | α-amino acid ester acyltransferase | Enzymatic production from AlaOMe and Gln | 320 mM (69.7 g/L) in 40 min | [32] |
E. coli OPA | None | α-amino acid ester acyltransferase | Enzymatic production from AlaOMe and Gln | 367.9 mM (79.8 g/L) in 20 min | [18] |
E.coli p15/AQ10 | ΔglsAΔglsBΔlpxMΔglnBΔglnEΔpepAΔpepBΔpepDΔpepNΔdpp | l-amino acid α-ligase (Lal/BacD) and glutamine synthetase (GlnA) | Whole-cell biocatalytic conversion of glutamic acid and alanine | 71.7 mM (14.2 g/L) in test tube in 18 h | This study |