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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Metabolic engineering of Escherichia coli for efficient production of l-alanyl-l-glutamine

Fig. 6

Regulation of the two synthesis modules by balancing the protein expression. a Production of AQ in whole-cell bioconversions using engineered strains with bacD and CgglnA expressed different order. The abbreviations are: p11, pYB1s-CgglnA-BsbacD; p12, pYB1s-BsbacD-CgglnA; p13, pYB1s-CgglnA-BabacD; p14, pYB1s-BabacD-CgglnA. p11, p12, p13, and p14 were individually introduced into strain AQ10. The engineered strains were induced and suspended in a reaction mixture containing 100 mM sodium glutamate, 100 mM alanine, 200 mM ammonium chloride, and 10 mM magnesium chloride. The bioconversion reactions were performed at 30 °C and 200 rpm for 18 h. b Extracellular AQ titer produced by strains with different RBS in the front of BsbacD CDS. The reaction was performed in a reaction mixture containing 100 mM sodium glutamate, and 100 mM alanine for 6 h. CK, p11/AQ10. c Modular expression of CgglnA and bacD genes in different order in a ara-operon configuration

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