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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Metabolic engineering of Escherichia coli for efficient production of l-alanyl-l-glutamine

Fig. 4

Extracellular glutamine titers produced by engineered strains overexpressing GlnA. WT wild type; BW, AQ02, AQ04, and AQ06 were transformed with plasmid p00. The engineered strains were induced and suspended in a reaction mixture containing 50 mM sodium glutamate, 10 mM magnesium chloride, and 100 mM ammonium chloride. The bioconversion reactions were performed at 30 °C and 200 rpm for 18 h. Glucose was supplemented at a concentration of 10 mM every 3 h

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