Fig. 4From: Metabolic engineering of Escherichia coli for efficient production of l-alanyl-l-glutamineExtracellular glutamine titers produced by engineered strains overexpressing GlnA. WT wild type; BW, AQ02, AQ04, and AQ06 were transformed with plasmid p00. The engineered strains were induced and suspended in a reaction mixture containing 50 mM sodium glutamate, 10 mM magnesium chloride, and 100 mM ammonium chloride. The bioconversion reactions were performed at 30 °C and 200 rpm for 18 h. Glucose was supplemented at a concentration of 10 mM every 3 hBack to article page