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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Metabolic engineering of Escherichia coli for efficient production of l-alanyl-l-glutamine

Fig. 2

Extracellular titers of AQ produced by engineered strains overexpressing BsBacD. WT wild type; BW, AQ06, AQ09, AQ10 were transformed with plasmid p01. The engineered strains were induced and suspended in a reaction mixture containing 50 mM glutamine, 50 mM alanine, and 10 mM magnesium chloride. The bioconversion reactions were performed at 30 °C and 200 rpm for 18 h. Glucose was supplemented at a concentration of 10 mM every 3 h

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