Fig. 2From: Metabolic engineering of Escherichia coli for efficient production of l-alanyl-l-glutamineExtracellular titers of AQ produced by engineered strains overexpressing BsBacD. WT wild type; BW, AQ06, AQ09, AQ10 were transformed with plasmid p01. The engineered strains were induced and suspended in a reaction mixture containing 50 mM glutamine, 50 mM alanine, and 10 mM magnesium chloride. The bioconversion reactions were performed at 30 °C and 200 rpm for 18 h. Glucose was supplemented at a concentration of 10 mM every 3 hBack to article page