Table 1 Strains and plasmids used in this study
From: Efficient production of myo-inositol in Escherichia coli through metabolic engineering
| Plasmid/strain | Description | References |
|---|---|---|
| Strains | ||
| E. coli transT1 | Wild-type | Invitrogen |
| E. coli BW25113 | Wild-type | Invitrogen |
| SG104 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs | Laboratory |
| R01 | E. coli BW25113,Δpgi | This study |
| R02 | E. coli BW25113,ΔpfkA | This study |
| R03 | E. coli BW25113,ΔpfkA,ΔpykF | This study |
| R04 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,Δpgi | This study |
| R05 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,ΔpfkA | This study |
| R06 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,ΔpfkA,ΔpykF | This study |
| R07 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,Δpgi,Δzwf | This study |
| R08 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,Δpgi,RBSL1-zwf | This study |
| R09 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,Δpgi,RBSL2-zwf | This study |
| R10 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,Δpgi,RBSL3-zwf | This study |
| R11 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,Δpgi,RBSL4-zwf | This study |
| R12 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,Δpgi,RBSL5-zwf | This study |
| R13 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,Δpgi,119 Promoter-zwf | This study |
| R14 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,Δpgi,Δpgm | This study |
| R15 | E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs,Δpgi,Δpgm,RBSL5-zwf | This study |
| Plasmids | ||
| pYB1s | p15A ori, pBAD promoter, StrR | Laboratory |
| pLB1a | R6K ori, pBAD promoter, AmpR | Laboratory |
| p01 | pYB1s containing TbIPS gene from Trypanosoma brucei | Laboratory |
| p02 | pLB1a containing EcIMP gene from E.coli | Laboratory |
| p03 | pLB1a containing ScIPS gene from saccharomyces cerevisiae | Laboratory |
| pR01 | pYB1s containing TbIPS-EcIMP genes | This study |
| pR02 | pYB1s containing EcIMP-TbIPS genes | This study |
| pR03 | pYB1s containing TbIPS-ScIPS-EcIMP genes | This study |