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Table 1 Strains and plasmids used in this study

From: Efficient production of myo-inositol in Escherichia coli through metabolic engineering

Plasmid/strain

Description

References

Strains

 E. coli transT1

Wild-type

Invitrogen

 E. coli BW25113

Wild-type

Invitrogen

 SG104

E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acs

Laboratory

 R01

E. coli BW25113,Δpgi

This study

 R02

E. coli BW25113,ΔpfkA

This study

 R03

E. coli BW25113,ΔpfkA,ΔpykF

This study

 R04

E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspgi

This study

 R05

E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspfkA

This study

 R06

E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspfkA,ΔpykF

This study

 R07

E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspgi,Δzwf

This study

 R08

E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspgi,RBSL1-zwf

This study

 R09

E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgi,RBSL2-zwf

This study

 R10

E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgi,RBSL3-zwf

This study

 R11

E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgi,RBSL4-zwf

This study

 R12

E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgi,RBSL5-zwf

This study

 R13

E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspgi,119 Promoter-zwf

This study

 R14

E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgipgm

This study

 R15

E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgipgm,RBSL5-zwf

This study

Plasmids

 pYB1s

p15A ori, pBAD promoter, StrR

Laboratory

 pLB1a

R6K ori, pBAD promoter, AmpR

Laboratory

 p01

pYB1s containing TbIPS gene from Trypanosoma brucei

Laboratory

 p02

pLB1a containing EcIMP gene from E.coli

Laboratory

 p03

pLB1a containing ScIPS gene from saccharomyces cerevisiae

Laboratory

 pR01

pYB1s containing TbIPS-EcIMP genes

This study

 pR02

pYB1s containing EcIMP-TbIPS genes

This study

 pR03

pYB1s containing TbIPS-ScIPS-EcIMP genes

This study