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Table 1 Strains and plasmids used in this study

From: Efficient production of myo-inositol in Escherichia coli through metabolic engineering

Plasmid/strainDescriptionReferences
Strains
 E. coli transT1Wild-typeInvitrogen
 E. coli BW25113Wild-typeInvitrogen
 SG104E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acsLaboratory
 R01E. coli BW25113,ΔpgiThis study
 R02E. coli BW25113,ΔpfkAThis study
 R03E. coli BW25113,ΔpfkA,ΔpykFThis study
 R04E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspgiThis study
 R05E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspfkAThis study
 R06E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspfkA,ΔpykFThis study
 R07E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspgi,ΔzwfThis study
 R08E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspgi,RBSL1-zwfThis study
 R09E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgi,RBSL2-zwfThis study
 R10E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgi,RBSL3-zwfThis study
 R11E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgi,RBSL4-zwfThis study
 R12E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgi,RBSL5-zwfThis study
 R13E. coli BW25113,ΔptsG::glk,ΔgalR::zglf,ΔpoxB::acspgi,119 Promoter-zwfThis study
 R14E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgipgmThis study
 R15E. coli BW25113,ΔptsG::glkgalR::zglfpoxB::acspgipgm,RBSL5-zwfThis study
Plasmids
 pYB1sp15A ori, pBAD promoter, StrRLaboratory
 pLB1aR6K ori, pBAD promoter, AmpRLaboratory
 p01pYB1s containing TbIPS gene from Trypanosoma bruceiLaboratory
 p02pLB1a containing EcIMP gene from E.coliLaboratory
 p03pLB1a containing ScIPS gene from saccharomyces cerevisiaeLaboratory
 pR01pYB1s containing TbIPS-EcIMP genesThis study
 pR02pYB1s containing EcIMP-TbIPS genesThis study
 pR03pYB1s containing TbIPS-ScIPS-EcIMP genesThis study