Fig. 6
From: Efficient production of myo-inositol in Escherichia coli through metabolic engineering
Scaled-up production of inositol by bioconversion by strain R04 ex situ (a) and in situ (b). The bioconversion ex situ was in a 1-L fermenter containing biomass (OD600 = 80), 1 mM MgSO4, and 1 × M9 salts buffer. Glucose was added in batches; 200 mM glucose was added at 0, 4.5, and 11 h respectively. The bioconversion in situ in a 1-L fermenter was started when the cells grew to the density of OD600 = 80. Glucose was added in batches; 200 mM glucose was added at 0, 3, 6, and 9 h, respectively