Fig. 5
From: Efficient production of myo-inositol in Escherichia coli through metabolic engineering
High-density fermentation of recombinant strains with high stoichiometric yields and bioconversion by strain R04. a High-density fermentation of strains R04, R12, R14, and R15. Cells were initially cultured in Luria–Bertani medium at 37 °C. Then, this seed culture was inoculated into 500 mL inorganic salts medium with glycerol and glucose as a mixed carbon source in a 1-L fermenter. b Comparison of bioconversion by strain R04 cultured in shaken flasks (1) and high-density fermentation (2). Cells were suspended in a mixture with 50 mM glucose for 10 h at 37 °C and 220 rpm