Table 2 Strains and plasmids used in this study
Strains and plasmids | Relevant gene type | Reference/source |
|---|---|---|
Strains | ||
 DH5α | E. coli F−Ф80lacZΔM15Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk− mk−) phoA supE44 thi−1gyrA96 relA1 | Lab stock |
 E. coli S17-1 (λpir) | Res− pro mod+ integrated copy of RP4, mob+, used for incorporating constructs into P. chlororaphis | Lab stock |
 BL21(DE3) | Host strain for pET28a | Invitrogen |
 HT66 | P. chlororaphis HT66 wild-type, Ampr Spr | This study |
 P3 | A mutant from HT66 with a high PCN production, Ampr Spr | This study |
 HT66ΔphzB | phzB in-frame deletion mutant of HT66 | This study |
 HT66ΔphzF | phzF in-frame deletion mutant of HT66 | This study |
 HT66ΔtrpE | trpE in-frame deletion mutant of HT66 | This study |
 HT66ΔNat | Nat in-frame deletion mutant of HT66 | This study |
 HT66ΔphzBΔtrpE | phzB, trpE double in-frame deletion mutant of HT66 | This study |
 HT66ΔphzBΔNat | phzB, Nat double in-frame deletion mutant of HT66 | This study |
 HT66ΔphzB-pBBRphz’-phzB | phzB complentation in HT66ΔphzB | This study |
 P3ΔphzB | phzB in-frame deletion mutant of P3 | This study |
 P3ΔphzBΔlonΔpykAΔrsmE | phzB, lon, pykA, rsmE in-frame deletion mutant of P3 | This study |
Plasmid | ||
 pK18mobsacB | Broad-host-range gene replacement vector, sacB, Kanr | Lab stock |
 pBBR1MCS | T7 expression vector, Kanr | Lab stock |
 PET-28a (+) | T7 promoter, Kanr, expression vector | Novagen |
 pBBR-phz’-phzB | Gene complementation vector, pBBR1MCS containing a 534 bp phz promoter fragment and the phzB gene cluster, Kanr | This study |
 PET28a (+)-Nat | pET28a (+) containing Nat | This study |