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Table 3 Key strains constructed and utilized in this study

From: Key factors affecting ammonium production by an Azotobacter vinelandii strain deregulated for biological nitrogen fixation

A. vinelandii strain

Genetic features

Plasmid utilized

Parent strain

DJ (ATCC BAA-1303)

Wild-type A. vinelandii with diminished alginate production resulting in ease of transformation

None

None

AZBB109

∆amtB

pPCRPYRF1a

AZBB106a

AZBB163b*

nifL::KanR(pPCRNH3-43). Was grown in medium without nitrogen source, and mutated to a nif+ phenotype that also produces high levels of extracellular ammonia

pPCRNH3-43, spontaneous mutation

AZBB150b

AZBB281*

∆amtB, nifL::KanR(pPCRNH3-44)

pPCRNH3-44c

AZBB109

  1. KanR Kanamycin resistance
  2. Strains indicated by an asterisk (*) are completed and were used in ammonium production experiments
  3. a As described previously [26]
  4. b As described previously [11]
  5. cAs described previously [6]