Key factors affecting ammonium production by an Azotobacter vinelandii strain deregulated for biological nitrogen fixation

Table 1 Key plasmid constructs utilized in this work

Plasmid^a	Relevant gene(s) cloned or plasmids manipulated	Parent vector	Source or references
pPCRNH3-44	Incorporated C to T mutation into kanamycin cassette region to create Nif⁺ phenotypes with high ammonium production	pPCRNH3-43	[6]
pPCRPYRF1	Cloned pyrF and flanking regions from A. vinelandii	pBB053	[26]

ISSN: 1475-2859