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Table 3 Comparison of parameters of each cycle for γ-PGA production by B. siamensis IR10 in repeated fed-batch fermentation

From: High-level production of poly-γ-glutamic acid from untreated molasses by Bacillus siamensis IR10

Cycle number

1

2

3

4

5

6

Time (h)

24

24

24

20

20

20

Biomass (g/L)

14.88 ± 0.49

15.88 ± 0.52

16.73 ± 0.55

16.77 ± 0.56

17.08 ± 0.57

17.09 ± 0.56

γ-PGA concentration (g/L)

40.06 ± 1.33

44.77 ± 1.39

45.42 ± 1.41

44.10 ± 1.23

44.90 ± 1.26

44.98 ± 1.26

γ-PGA productivity (g/L/h)

1.67 ± 0.05

1.70 ± 0.06

1.71 ± 0.06

1.98 ± 0.06

2.02 ± 0.06

2.02 ± 0.06

  1. The productivity was calculated according to the produced γ-PGA production as follows
  2. Cycle 1: 40.06 ÷ 24 = 1.67; Cycle 2: (44.77 − 4.00) ÷ 24 = 1.70; Cycle 3: (45.42 − 4.40) ÷ 24 = 1.71; Cycle 4: (44.10 − 4.54) ÷ 20 = 1.98
  3. Cycle 5: (44.90 − 4.53) ÷ 20 = 2.02; Cycle 6: (44.98 − 4.53) ÷ 20 = 2.02
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Microbial Cell Factories

ISSN: 1475-2859

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