Skip to main content
Fig. 5 | Microbial Cell Factories

Fig. 5

From: Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology

Fig. 5

Deletion of redundant sequences with CRISPR-Cas9 system. a PCR confirmation of the deletion of redundant sequences using primers IS5-check-P1 and IS5-check-P2. The decrescent bands indicated the successful deletion of redundant sequences. M: DNA marker; CK: strain without deletion of redundant sequences; 1,2,3,4,5,6,7,8,9: colonies from the plate after editing using CRISPR-Cas9 system. b The lycopene yields of shake flask fermentation of strain EC101, EC-IS5, EC401 and EC-IS5 (ΔCm). Each value represents the average ± SD of three biological replicates

Back to article page