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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology

Fig. 3

Promoters characterization of three modules for lycopene biosynthesis. a Selection promoters for lycopene synthetic modules (including crtI, crtE and crtB). Lycopene synthetic modules were overexpressed in E. coli W3110 with the native MEP pathway. trc, EC101; yciG, EC102; pstA, EC103; yodA, EC104; astC, EC105; ybiM, EC106. b Selection promoters for feedforward modules (including dxs and dxr). Feedforward modules were overexpressed along with lycopene synthetic modules. phnI, EC201; phoR, EC202; phnF, EC203; phnC, EC204; phnD, EC205. c Selection promoters for feedback modules (including idi and crtE). Feedback modules were overexpressed along with lycopene synthetic modules. yfiL, EC301; yijF, EC302; cysP, EC303; yeiG, EC304; yhcN, EC305. Each value represents the average ± SD of three biological replicates

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Microbial Cell Factories

ISSN: 1475-2859

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