Fig. 2
In vitro recombined of heterologous modules crtZ and crtW. a Workflow of the in vitro recombination to evolve the heterologous pathway of astaxanthin in yeast. The acceptor vector and the pool of donor constructs (crtZ and crtW) are mixed with Cre recombinase in vitro. The donor fragments will be randomly inserted into LoxPSym sites of the acceptor vector, assembled into various new plasmids. b The reaction products were transformed into yQDD001, and produced the yeast library with different colors and sizes. c Astaxanthin yield measurement of in vitro recombined strains. And the genotype of yQDD002–yQDD010 was proved by PCRTag analysis. The error bars represent standard deviations calculated from duplicate experiments. “Astaxanthin yield” was determined as “the astaxanthin content in single-cell” with the unit as mg g−1 DCW