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Table 1 Plasmids, oligonucleotides and strains used in this study

From: Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae

NameDescription or sequence (5′ to 3′)Source
Plasmids  
 pIDT_PETase_OPTCommercially distributed plasmid including codon-optimized PETase gene for expression in C. reinhardtiiThis study
 pBR9_mCherry_CreDeposited plasmid, containing codon optimized mCherry-encoding geneCRC
 pBR9_PETase_CrepBR9 containing PETase-encoding gene for C. reinhardtii from pBR9_mCherry_CreThis study
Oligonucleotides  
 Cre_Sh-ble_PETase FGCAACTGCGTGCACTTCGTThis study
 Cre_Sh-ble_PETase RTTCTCGCAAGCAAAGATCAGCGTThis study
Strains  
 E. coli DH5α RBC bioscience
 C. reinhardtii CC-124C. reinhardtii wild type strain carrying nit1 and nit2 mutationCRC
 C. reinhardtii CC-503Cell-wall-less mutant originated from C. reinhardtii CC-125CRC
 C. reinhardtii CC-124 #1 to #11C. reinhardtii CC-124 transformants harboring PETase expression cassetteThis study
 C. reinhardtii CC-503 #12 to #25C. reinhardtii CC-503 transformants harboring PETase expression cassetteThis study