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Table 1 Plasmids, oligonucleotides and strains used in this study

From: Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae

Name

Description or sequence (5′ to 3′)

Source

Plasmids

  

 pIDT_PETase_OPT

Commercially distributed plasmid including codon-optimized PETase gene for expression in C. reinhardtii

This study

 pBR9_mCherry_Cre

Deposited plasmid, containing codon optimized mCherry-encoding gene

CRC

 pBR9_PETase_Cre

pBR9 containing PETase-encoding gene for C. reinhardtii from pBR9_mCherry_Cre

This study

Oligonucleotides

  

 Cre_Sh-ble_PETase F

GCAACTGCGTGCACTTCGT

This study

 Cre_Sh-ble_PETase R

TTCTCGCAAGCAAAGATCAGCGT

This study

Strains

  

 E. coli DH5α

 

RBC bioscience

 C. reinhardtii CC-124

C. reinhardtii wild type strain carrying nit1 and nit2 mutation

CRC

 C. reinhardtii CC-503

Cell-wall-less mutant originated from C. reinhardtii CC-125

CRC

 C. reinhardtii CC-124 #1 to #11

C. reinhardtii CC-124 transformants harboring PETase expression cassette

This study

 C. reinhardtii CC-503 #12 to #25

C. reinhardtii CC-503 transformants harboring PETase expression cassette

This study