Table 3 Physico-chemical characterization of E20c

Untreated control

16 ± 0.11

Temperature

60 °C (30 min)

16 ± 0.12

60 °C (60 min)

14 ± 0.10

80 °C (30 min)

14 ± 0.22

80 °C (60 min)

14 ± 0.08

100 °C (15 min)

14 ± 0.14

100 °C (30 min)

13 ± 0.15

100 °C (60 min)

13 ± 0.15

121 °C (15 min)

–*

pH

2

10 ± 0.17

4

16 ± 0.11

6

16 ± 0.04

8

13 ± 0.24

10

–

Enzymes (1 mg/ml)

Proteinase K

–

Pepsin

–

Trypsin

–

Lipase

16 ± 0.12