Table 1 Strains and plasmids used in this study

C. glutamicum strains

 DelAro⁴-4cl_Pc C5 mufasO_BCD1 P_O6-iolT1 Δpyc (M-CoA)

C. glutamicum derivative with in-frame deletions of cg0344-47, cg0503 cg2625-40 and cg1226; harboring a chromosomally encoded codon-optimized 4cl_Pc gene coding for 4-coumarate:CoA ligase from P. crispum under control of the T7 promoter and replacement of the native gltA promotor with the dapA promotor variant C5, mutated fasO binding sites upstream of accBC and accD1, two nucleotide exchanges in the iolT1 promotor and in-frame deletion of pyc

[16]

 M-CoA ΔldhA

C. glutamicum M-CoA derivative with in-frame deletion of ldhA

This work

E. coli strains

 DH5α

F^–Φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (r^–_K,m⁺_K) phoA supE44 λ^– thi-1 gyrA96 relA1

Invitrogen (Karlsruhe, Germany)

Plasmids

 pK19mobsacB-ΔldhA

Vector for in-frame deletion of ldhA

[46]

 pMKEx2

kan^r; E. coli-C. glutamicum shuttle vector (lacI, P_T7, lacO1, pHM1519 ori_Cg; pACYC177 ori_Ec)

[21]

 pMKEx2-bas_RpCg-rzs1_RiCg

kan^r; pMKEx2 derivative containing codon-optimized genes encoding benzalacetone synthase from R. palmatum (bas_RpCg) and NADPH-dependent raspberry ketone/zingerone reductase from R. idaeus (rzs1_RiCg)

This work

 pMKEx2-bas_RpCg-rzs1_RiCg-G191D

kan^r; pMKEx2-bas_RpCg-rzs1_RiCg derivative with mutations in the rzs1_RiCg nucleotide sequence leading to amino acid substitution G191D in RZS1_Ri.

This work

 pMKEx2-bas_RpCg-curA_Ec

kan^r; pMKEx2 derivative containing a codon-optimized gene encoding benzalacetone synthase from R. palmatum (bas_RpCg) and the native gene encoding NADPH-dependent curcumin/dihydrocurcumin reductase CurA from E. coli (curA_Ec)

This work

 pMKEx2-bas_RpCg-curA_EcCg

kan^r; pMKEx2 derivative containing codon-optimized genes encoding benzalacetone synthase from R. palmatum (bas_RpCg) and NADPH-dependent curcumin/dihydrocurcumin reductase CurA from E. coli (curA_EcCg)

This work

 pMKEx2-pks1_RiCg-curA_EcCg

kan^r; pMKEx2 derivative containing codon-optimized genes encoding PKS1 from R. idaeus (pks1_RiCg) and NADPH-dependent curcumin/dihydrocurcumin reductase CurA from E. coli (curA_EcCg)

This work

 pMKEx2-pks4_RiCg-curA_EcCg

kan^r; pMKEx2 derivative containing codon-optimized genes encoding PKS4 from R. idaeus (pks4_RiCg) and NADPH-dependent curcumin/dihydrocurcumin reductase CurA from E. coli (curA_EcCg)

This work

 pMKEx2-malE_Ec-pks1_RiCg-curA_EcCg

kan^r; pMKEx2 derivative containing the native malE gene from E. coli fused to the codon-optimized gene encoding PKS1 from R. idaeus (malE_Ec-pks1_RiCg) and a codon-optimized gene encoding NADPH-dependent curcumin/dihydrocurcumin reductase CurA from E. coli (curA_EcCg)

This work

 pMKEx2-malE_Ec-pks4_RiCg-curA_EcCg

kan^r; pMKEx2 derivative containing the native malE gene from E. coli fused to the codon-optimized gene encoding PKS4 from R. idaeus (malE_Ec-pks4_RiCg) and a codon-optimized gene encoding NADPH-dependent curcumin/dihydrocurcumin reductase CurA from E. coli (curA_EcCg)

This work

 pEKEx3

spec^r; E. coli-C. glutamicum shuttle vector (lacI,P_tac, lacO1, pBL1ori_Cg; pUCori_Ec)

[47]

 pEKEx3-pntAB_Ec

spec^r; pEKEx3 derivative containing native pntAB genes from E. coli (pntAB_Ec) encoding a membrane-bound transhydrogenase

This work

 pEKEx3-udhA_EcCg

spec^r; pEKEx3 derivative containing codon-optimized udhA gene variant from E. coli (udhA_EcCg) encoding a cytoplasmatic transhydrogenase

This work

 pEKEx3-malE_Ec-omt_Vv

spec^r; pEKEx3 derivative containing malE gene from E. coli (malE_Ec) fused to the codon-optimized gene coding for resveratrol-di-O-methyltransferase from V. vinifera (omt_Vv)

[30]