Fig. 5

Combinatorial biosynthesis of phenylbutanoids zingerone and benzylacetone from supplemented phenylpropanoid- and diketide precursors using C. glutamicum M-CoA ΔldhA pMKEx2-bas_RpCg-curA_EcCg pEKEx3-udhA_EcCg. Cultivations were performed in 50 mL CGXII medium with 4% glucose and either 3.09 mM diketide or 5 mM phenylpropanoid precursors in baffled flasks at 30 °C and 130 rpm for 72 h. Here, supplementation of p-coumaric acid and p-hydroxybenzalacetone for the synthesis of RK served as a positive control. a Microbial growth and b phenylbutanoid synthesis from supplemented diketides. c Microbial growth and d phenylbutanoid synthesis from supplemented phenylpropanoids. Where appropriate, determined concentrations for diketides (filled bars) and phenylbutanoids (checkered bars) were stacked for a better data visualization. Depicted data represent mean values with standard deviations from biological triplicates