Fig. 4

Evaluation of different benzalacetone synthases for raspberry ketone production from p-coumaric acid using C. glutamicum. C. glutamicum M-CoA ΔldhA harboring the indicated expression plasmids was cultivated in 50 mL CGXII medium with 4% glucose and 5 mM p-coumaric acid in baffled flasks at 30 °C and 130 rpm for 72 h. a Growth and bp-hydroxybenzalacetone- and raspberry ketone synthesis was followed over time. Determined concentrations for p-hydroxybenzalacetone and raspberry ketone in cell extracts were stacked for a better visualization. The depicted data represent mean values with standard deviations from biological triplicates