Repeated fed-batch strategy and metabolomic analysis to achieve high docosahexaenoic acid productivity in Crypthecodinium cohnii

Table 3 Comparison of DHA production of each cycle in repeated fed-batch culture of four cycles

Repeated fed-batch	Fermentation time (h)	Dry cell weight (g/L)	DHA (g/L)	DHA productivity (mg/L/h)	Fatty acid composition (%)^a
Repeated fed-batch	Fermentation time (h)	Dry cell weight (g/L)	DHA (g/L)	DHA productivity (mg/L/h)	C12:0	C14:0	C16:0	C18:0	C18:1	C22:6
Cycle 1	120	46.80 ± 0.87	6.18 ± 0.01	51.54 ± 0.06	3.08 ± 0.36	14.28 ± 1.13	18.77 ± 0.56	2.58 ± 0.36	12.18 ± 0.18	49.12 ± 2.04
Cycle 2	120	45.98 ± 0.24	6.05 ± 0.03	50.43 ± 0.22	3.74 ± 0.26	14.00 ± 0.20	19.45 ± 0.18	2.19 ± 0.03	11.61 ± 0.23	49.02 ± 0.50
Cycle 3	120	46.48 ± 0.29	6.11 ± 0.06	50.94 ± 0.54	3.88 ± 0.04	12.95 ± 0.84	19.16 ± 0.93	2.85 ± 0.36	12.09 ± 0.36	49.06 ± 1.48
Cycle 4	120	44.76 ± 0.08^*	5.88 ± 0.08	48.97 ± 0.64	3.58 ± 0.35	12.82 ± 0.58	20.25 ± 0.38	3.43 ± 0.73	11.40 ± 0.34	48.53 ± 0.49

^aLauric acid (C12:0). Myristic acid (C14:0). Palmitic acid (C16:0). Stearic acid (C18:0). Oleic acid (C18:1). Docosahexaenoic acid (C22:6)
* Significant differences between cycle 4 and cycle 1 evaluated by a statistical Student’s T-test (*p < 0.05)

ISSN: 1475-2859