Fig. 4
From: CRISPR/Cas9-deaminase enables robust base editing in Rhodobacter sphaeroides 2.4.1
ABEs were lowly or mediumly efficient and caused specific A-G mutation after streak. a The diagrammatic sketch of main components in ABEs. b The transformation efficiencies of dABE and nABE series single targets, each with 0 mM or 0.5 mM IPTG induction, respectively. c The sequencing map of appA0 (upper panel), ppsR0 (middle panel), and bchG0 (below panel) target, with the first induction (transform) and the second induction (streak). The red colored As meant the editable As in the target, and the underlined triplet nucleotides were the start codon for each gene. Black arrows indicated the position where the mutation peaks of A appeared, and the fractional number on the right was the corresponding screening rate. d The percentage of good (G) and moderate (G≥A) mutants for dABE or nABE series single targets, respectively. e The hot position of A and its mutation frequency for dABE or nABE series single targets, respectively