Recombinant protein production associated growth inhibition results mainly from transcription and not from translation

Table 2 Primers for qPCR

Gene	Primer sequence 5′–3′	Tm (°C)	Amplicon (bp)
rrsH	Fw: TCAGCGGGGATGAAGGGAGTAAAG	58.0	209
	Rev: GCCAGTATCAGATGCAGTTCCCAG	57.4
tufA	Fw: TTCTTCAAAGGCTACCGTCCGC	57.1	211
	Rev: GAACTTTAGCAACAACGCCCGC	58.1
hFGF-2	Fw: AAGACCCGAAACGTCTGTACTGC	57.1	215
	Rev: GGTAACGCATTTGGAAGCCAGC	57.1
GFP	Fw: TGGAAAACTACCTGTTCCCTGGC	56.6	198
	Rev: CCTTCAAACTTGACTTCAGCACGC	57.3

ISSN: 1475-2859