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Table 1 Plasmids

From: Recombinant protein production associated growth inhibition results mainly from transcription and not from translation

Plasmids

Features

pET-28c/pET-29c

Novagen, Germany

pET-28cΔRBS

Mutant plasmid with deletion of all RBSs (AAGGAG) between T7 promoter and T7 terminator

pET-29c-hFGF-2

Parental plasmid for the production of hFGF-2 [15]

pET-29c-hFGF-2 ΔRBS

Mutant plasmid with deletion of all RBSs (AAGGAG) between T7 promoter and T7 terminator

pET-29c-hFGF-2 ΔT7

Mutant plasmid with deletion of T7 promoter region (TAATACGACTCACTATAGG)

pET-29c-hFGF-2 ΔRBS & ΔT7

Mutant plasmid with deletion of all RBSs (AAGGAG) between T7 promoter and T7 terminator as well as T7 promoter region (TAATACGACTCACTATAGG)

pET-28c-His6-GFP

Parental plasmid for the production of GFP [21]

pET-28c-His6-GFP ΔRBS

Mutant plasmid with deletion of all RBSs (AAGGAG) between T7 promoter and T7 terminator