Fig. 8

Growth properties of E. coli BL21 (DE3) carrying the “empty” pET-28c expression vector. Cells carrying the original “empty” pET-28c expression vector with promoter region and RBS (circles) and the “empty” vector with promoter region but without RBS (diamonds) were grown in shake flask cultures in Luria–Bertani medium. Growth of cells carrying the original expression vector encoding hFGF-2 with promoter region and with RBS is revisited for a better comparison (broken lines, open squares). Black symbols always correspond to IPTG induced cultures and gray symbols to cultures where IPTG addition was omitted. The time-point of IPTG addition to a final concentration of 0.5 mM is indicated by an arrow. Data are presented as mean ± SD, n = 4