Fig. 6

GFP only forms inclusion bodies during production on defined medium. SDS-PAGE analysis of IPTG induced cells carrying the gene encoding GFP growing on LB medium (lanes 1–6) and glucose supplemented mineral salt medium (lanes 8–13) collected 4 h after induction. Lanes 1, 10: N, T7⁺ RBS⁺ (non-induced controls, total cell extract of cells containing the expression vector with promoter and with RBS in front of the GFP gene), lanes 2, 11: W, T7⁺ RBS⁺ (induced cells with promoter and with RBS), lanes 3, 12: N, T7⁺ ΔRBS (non induced controls with promoter but without RBS), lanes 4, 13: W, T7⁺ ΔRBS (induced cells with promoter but without RBS), lanes 5, 8: S, T7⁺ RBS⁺ (soluble) and lanes 6, 9: I, T7⁺ RBS⁺ (insoluble cell fraction of induced cells containing the expression vector with promoter and with RBS). Lane 7: molecular mass marker, the position of GFP is indicated by an arrow. N: non-induced whole cell protein W: induced whole cell protein, S: induced soluble part, and I: induced insoluble part of whole cell protein