Impact of lignans in oilseed mix on gut microbiome composition and enterolignan production in younger healthy and premenopausal women: an in vitro pilot study

Table 1 Multiple reaction monitoring (MRM) detection parameters

Compound	MW	Parent m/z	fr m/z	CV	CE	Equation	R²	LOD	LOQ
Seco	362.42	361.4	122^a	30	30	y = 5.5627x	0.9989	0.025571	0.085237
Seco	362.42	361.4	346		17
Lari	360.4	359.4	329^a	30	20	y = 20.963x	0.991	0.009145	0.030483
Lari	360.4	359.4	192		10
Pino	358.39	357.4	151^a	30	17	y = 9.5455x + 261.14	0.9914	0.011945	0.039818
Pino	358.39	357.4	136		30
Mat	302.36	357.42	83^a	30	23	y = 33.014x + 1235.7	0.9942	0.013987	0.046623
Mat	303.36	357.42	137		20
ED	298.33	301	253^a	30	20	y = 67.712x + 5969.6	0.9912	0.111075	0.370249
ED	298.33	301	271		20
EL	298.33	297.3	107^a	30	25	y = 105.71x + 17165	0.9984	0.019281	0.06427
EL	298.33	297.3	189		20
Seco d⁶	368.45	367	168^a`	30	25	y = 4.2692x − 60.534	0.9985	0.033394	0.111314
Seco d⁶	368.45	367	124		29

MW Molecular weight, fr fragment; ^a quantification fragment; CV collision voltage, CE collision energy, LOD limit of detection; LOQ limit of quantification. Calibration curves showed good linearity, LOD was calculated as a signal to noise ratio (S/N) equal to 3; LOQ was calculated as a signal to noise ratio (S/N) equal to 10

ISSN: 1475-2859