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Table 2 Nucleotide sequences of the TIRUNEVOLVED and corresponding TIREVOLVED used in this study. The TIR is defined as the region from the Shine-Dalgarno to codon five of the signal peptide

From: Increased production of periplasmic proteins in Escherichia coli by directed evolution of the translation initiation region

  1. 1Underlined region was randomised during the directed evolution process
  2. 2Nucleotides marked in bold text were changed in TIRSYN_EVOLVED
  3. 3Shine-Dalgarno sequence marked in blue 4Start methionine (ATG) marked in grey
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Microbial Cell Factories

ISSN: 1475-2859

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