Table 1 The production of Streptomyces trypsin in different strains

S. lividans 1326

6.6 U mL⁻¹

pWHM3- sprT, recombinant expression

[55]

S. lividans TK24

7.4 U mL⁻¹

Recombinant expression

[56]

S. griseus IFO13350 (pWHM3-TR1R2)

12.84 U mL⁻¹

Co-expressing positive regulatory genes sgtR1, sgtR2; medium optimization (5.5-fold increased)

[19]

S. griseus IFO13350 (pWHM3-TR1R2)

15.18 U mL⁻¹

Expression vectors containing sprT, sgtR1, and sgtR2

[18]

E. coli

–

–

[16]

Bacillus subtilis SCK6

33.8 U mL⁻¹

Streptomyces trypsin GM2938

[30]

Pichia pastoris

14.4 U mL⁻¹

Mature SGT sequence; propeptide swap; fed-batch fermentation

[20]

P. pastoris

1.45 U mL⁻¹

Propeptide engineering; 6.71-fold increased (flask culture)

[21]

P. pastoris

47.4 U mL⁻¹

Auto-catalyzed N-terminal peptide; fed-batch fermentation

[22]

P. pastoris GS115-tbcf (101A)*

40.5 U mL⁻¹

Pro-peptide mutation; artificial pro-peptide; single mutation of autolysis residues (flask culture)

[13]

P. pastoris GS115-tbcf (101A)

227.65 U mL⁻¹

Fed-batch fermentation

[13]

GS115-tbcf (K101A, R201V)

60.85 U mL⁻¹

tbcf (K101A, R201V) mutant, 1.5-fold increased (flask culture)

In study

GS115-tbcf (K101A, R201V)-UBC1

80.1 U mL⁻¹

Overexpression UBC1, 1.98-fold increased (flask culture)

In study

GS115-tbcf (K101A, R201V)-SSO2

86.12 U mL⁻¹

Overexpression SSO2, 2.13-fold increased (flask culture)

In study

GS115-tbcf (K101A, R201V)-SU

109.25 U mL⁻¹

Overexpression SSO2 and UBC1, 2.7-fold increased (flask culture)

In study

GS115-tbcf (K101A, R201V)-SU

689.47 U mL⁻¹

Fed-batch fermentation, threefold increased from GS115-tbcf (101A)

In study