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Table 1 The production of Streptomyces trypsin in different strains

From: Improving production of Streptomyces griseus trypsin for enzymatic processing of insulin precursor

StrainsProductionStrategiesReferences
S. lividans 13266.6 U mL−1pWHM3- sprT, recombinant expression[55]
S. lividans TK247.4 U mL−1Recombinant expression[56]
S. griseus IFO13350 (pWHM3-TR1R2)12.84 U mL−1Co-expressing positive regulatory genes sgtR1, sgtR2; medium optimization (5.5-fold increased)[19]
S. griseus IFO13350 (pWHM3-TR1R2)15.18 U mL−1Expression vectors containing sprT, sgtR1, and sgtR2[18]
E. coli[16]
Bacillus subtilis SCK633.8 U mL−1Streptomyces trypsin GM2938[30]
Pichia pastoris14.4 U mL−1Mature SGT sequence; propeptide swap; fed-batch fermentation[20]
P. pastoris1.45 U mL−1Propeptide engineering; 6.71-fold increased (flask culture)[21]
P. pastoris47.4 U mL−1Auto-catalyzed N-terminal peptide; fed-batch fermentation[22]
P. pastoris GS115-tbcf (101A)*40.5 U mL−1Pro-peptide mutation; artificial pro-peptide; single mutation of autolysis residues (flask culture)[13]
P. pastoris GS115-tbcf (101A)227.65 U mL−1Fed-batch fermentation[13]
GS115-tbcf (K101A, R201V)60.85 U mL−1tbcf (K101A, R201V) mutant, 1.5-fold increased (flask culture)In study
GS115-tbcf (K101A, R201V)-UBC180.1 U mL−1Overexpression UBC1, 1.98-fold increased (flask culture)In study
GS115-tbcf (K101A, R201V)-SSO286.12 U mL−1Overexpression SSO2, 2.13-fold increased (flask culture)In study
GS115-tbcf (K101A, R201V)-SU109.25 U mL−1Overexpression SSO2 and UBC1, 2.7-fold increased (flask culture)In study
GS115-tbcf (K101A, R201V)-SU689.47 U mL−1Fed-batch fermentation, threefold increased from GS115-tbcf (101A)In study
  1. –, Inclusion body
  2. * The parent strain in this study