Fig. 6
From: Improving production of Streptomyces griseus trypsin for enzymatic processing of insulin precursor
HPLC chromatograms of insulin manufactured with trypsin. a Schematic presentation of the hydrolysis process. rPI, recombination insulin precursor; PI-BD30, insulin precursor with Asp30-deleted B-chain. b–d Chromatograms of the hydrolysis products of rPI after incubation with trypsin at 25 °C for 19 h: b rPI, c rPI and commercial porcine trypsin, and d rPI and tbcf (K101A, R201V)