Table 2 Kinetic constants of EstM2
Phthalate ester | Vmax (µmol mg −1min−1) | Km (mM) | kcat/Km (mM−1 s−1) |
|---|---|---|---|
Dimethyl phthalate (DMP) | 42.14 ± 3.3 | 1.02 ± 0.08 | 29.63 ± 2.3 |
Diethyl phthalate (DEP) | 58.98 ± 3.8 | 0.80 ± 0.05 | 52.84 ± 3.4 |
Di-n-butyl phthalate (DBP) | 72.87 ± 4.4 | 0.62 ± 0.04 | 84.23 ± 5.1 |
Butyl benzyl phthalate (BBP) | 57.58 ± 3.4 | 0.64 ± 0.04 | 64.48 ± 3.8 |
Diphenyl phthalate (DPP) | 45.10 ± 3.0 | 0.59 ± 0.04 | 54.78 ± 3.6 |
Monobenzyl phthalate (MBzP) | 43.78 ± 3.2 | 1.12 ± 0.08 | 28.02 ± 2.0 |
Monobutyl phthalate (MBP) | 56.67 ± 3.9 | 1.26 ± 0.08 | 32.24 ± 2.2 |
Monomethyl phthalate (MMP) | 41.28 ± 3.2 | 1.95 ± 0.15 | 15.17 ± 1.2 |
Monoethyl phthalate (MEP) | 54.21 ± 4.0 | 1.73 ± 0.13 | 22.46 ± 1.6 |
Monophenyl phthalate (MPP) | 37.59 ± 2.8 | 1.06 ± 0.08 | 25.42 ± 1.9 |
p-nitro phenyl acetate (C2) | 747.9 ± 18.8 | 0.41 ± 0.01 | 1292.5 ± 32.5 |
p-nitro phenyl butyrate (C4) | 905.0 ± 21.2 | 0.44 ± 0.01 | 1462.1 ± 34.2 |
p-nitro phenyl hexanoate (C6) | 71.39 ± 4.2 | 0.28 ± 0.02 | 182.78 ± 10.7 |