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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Characterization of a novel family VIII esterase EstM2 from soil metagenome capable of hydrolyzing estrogenic phthalates

Fig. 2

a Phylogenetic analysis of EstM2 with reported family VIII esterases. Numbers at the nodes indicate the levels of bootstrap support based on neighbor joining analysis of 100 resampled data sets. Bootstrap values below 50% are not shown. The scale bar represents 0.1 substitutions per nucleotide position. GenBank accession numbers are indicated within parentheses. AmpC, a class C β-lactamase from Aeromonas enteropelogenes (ABW05394) was used as an outgroup. b Multiple sequence alignment of EstM2 and other family VIII carboxylesterases showing the conserved sequence motifs. Amino acid residues responsible for the formation of catalytic triad for ester bond hydrolysis are shaded. Protein sequences with classical pentapeptide G-x-S-x-G motif are shown within a box. Alignment of two class C β-lactamases from Aeromonas enteropelogenes (AmpC, ABW05394) and Enterobacter cloacae (Blac, Q59401) respectively were also shown for sequence comparisons

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Microbial Cell Factories

ISSN: 1475-2859

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