Fig. 3From: Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of kasB in the cell wall synthesis of Mycobacterium neoaurumEffects of kasB on the component of cell wall mycolic acids in M. neoaurum. a The strain carrying the wild type kasB (Mn) or the deficient kasB (MnΔkasB) was cultivated in the presence of 1.0 g/L phytosterols. MAMEs (α-, methoxy- and keto- forms of mycolic acids) were isolated from M. neoaurum cells. TLC plates were revealed with cupric sulfate (10% w/v in an 8% v/v phosphoric acid solution). b Relative intensity of the mycolate compared to the total mycolates was calculated. The deletion of kasB caused a slight disturbance of MAMEs components in MnΔkasB (α-: 25.1%, methoxy-: 23.5%, and keto-: 51.4%) compared with that of the Mn strain (α-: 23.5%, methoxy-: 22.6%, and keto-: 53.9%)Back to article page