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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of kasB in the cell wall synthesis of Mycobacterium neoaurum

Fig. 3

Effects of kasB on the component of cell wall mycolic acids in M. neoaurum. a The strain carrying the wild type kasB (Mn) or the deficient kasB (MnΔkasB) was cultivated in the presence of 1.0 g/L phytosterols. MAMEs (α-, methoxy- and keto- forms of mycolic acids) were isolated from M. neoaurum cells. TLC plates were revealed with cupric sulfate (10% w/v in an 8% v/v phosphoric acid solution). b Relative intensity of the mycolate compared to the total mycolates was calculated. The deletion of kasB caused a slight disturbance of MAMEs components in MnΔkasB (α-: 25.1%, methoxy-: 23.5%, and keto-: 51.4%) compared with that of the Mn strain (α-: 23.5%, methoxy-: 22.6%, and keto-: 53.9%)

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