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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of kasB in the cell wall synthesis of Mycobacterium neoaurum

Fig. 2

Effects of the deficiency of kasB on the cell permeability. a Validation of allelic replacement at the kasB locus in M. neoaurum ATCC 25795. The wild type (WT) 3260-bp was replaced by a 2282-bp fragment ligate with the upstream and downstream homologous arm. b Growth characteristic of the kasB mutant strain. The wide-type M. neoaurum (Mn), the kasB-deficient strain (MnΔkasB) and the kasB-complemented strain (MnΔkasB + kasB) were cultured in MM containing 1.0 g/L cholesterol. c Determination of the cell permeability in the kasB mutant strain. The cells were stained with FDA, incubated at 32 °C for 10 min, and analyzed by a fluorescence spectrophotometer. The mutant strain MnΔkasB displayed about two times penetrated FDA compared with that in its parental Mn strain after 30 min of incubation. d Influences of the deficiency of kasB on the steroid (cholest-4-en-3-one) uptake. The cholest-4-en-3-one entering the cells after 12-h growth in MM containing 1.0 g/L cholest-4-en-3-one was determined. The uptake of cholest-4-en-3-one in the strain MnΔkasB showed about 2.3 times improvement than that of the wild type Mn strain

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