Table 1 Strains and plasmids used in the study
Name | Description | References |
|---|---|---|
WT-D | Citric acid producing strain isolated from soil sample | Lab store |
D353 | Derived from WT-D after Co60 γ- ray radiation mutagenesis | Lab store |
D10 | kusA::hph, pyrG::hph, hygR | This study |
D12 | pyrG::hph, hygR | This study |
D13 | pyrG::hph, hygR | This study |
D14 | pyrG::hph, hygR | This study |
D20 | pyrG::hph, hygR | This study |
D353.4 | pyrG::hph, hygR | This study |
D353.8 | kusA::hph, pyrG::hph, hygR | This study |
D353.10 | pyrG::hph, hygR | This study |
D353.18 | pyrG::hph, hygR | This study |
D353.20 | pyrG::hph, hygR | This study |
XMD1.6 | PpyrG::Tet-on, hygR | This study |
pSM-AnpyrG | PtrpC:AnpyrG:TtrpC | This study |
pCas9-hph | PglaA:nls-Cas9-nls:TglaA, hygR | This study |
pCas9-AnpyrG | PglaA:nls-Cas9-nls:TglaA, pyrGR | This study |
psgRNA6.1 | P5S rRNA:sgRNA-albA:Tpoly(T)6 | [10] |
psgRNA6.13 | P5S rRNA:sgRNA-kusA:Tpoly(T)6 | This study |
psgRNA6.14 | P5S rRNA:sgRNA-pyrG1:Tpoly(T)6 | This study |
psgRNA6.15 | P5S rRNA:sgRNA-pyrG2:Tpoly(T)6 | This study |