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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Disruption or reduced expression of the orotidine-5′-decarboxylase gene pyrG increases citric acid production: a new discovery during recyclable genome editing in Aspergillus niger

Fig. 2

Citric acid fermentation characteristics of the pyrG disrupted mutants. a, b citric acid titer and c, d normalized citric acid titer (g citric acid/g dry weight) were calculated for each strain. 1 × 105 spores/mL were inoculated in 20 mL citrate fermentation (CitFM) media and incubated at 34 °C for 96 h. The extracellular citric acid was determined by the method of HPLC. WT-D and D353, the parent strains used as the control; D10, D12, D13, D14, and D20, the pyrG deficient mutants derived from WT-D; D353.4, D353.8, D353.10, D353.18, D353.20, the pyrG deficient mutants derived from D353. Among them, D10, D20 and D353.8 were the pyrG and kusA double deficient mutants. Pairwise Student’s t-tests were conducted between conditional expression mutant relative to the parent strains. p values are indicated as (< 0.05, *; < 0.01, **; < 0.001, ***)

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Microbial Cell Factories

ISSN: 1475-2859

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