Fig. 4
From: Production of drug metabolites by human FMO3 in Escherichia coli
The superimposed HPLC chromatograms of GSK5182 (blue trace) and its conversion to its N-oxide by human FMO3 (red trace). The enzyme shows regioselectivity only metabolizing the Z-isomer (retention time of 5.5 min), while the E-isomer is untouched (retention time of 7.5 min). The N-oxide product (retention time of 9.3 min) was identified by LC–MS