Skip to main content

Table 1 Plasmids and strains used in the present study

From: Synthesis of acridone derivatives via heterologous expression of a plant type III polyketide synthase in Escherichia coli

Plasmids or E. coli strain

Relevant properties or genetic marker

Source or reference

Plasmids

 pACYCDuet-1

P15A ori, Cmr

Novagen

 pCDFDuet-1

CloDE13 ori, Strr

Novagen

 pETDuet-1

f1 ori, Ampr

Novagen

 pRSFDuet-1

RSF ori, Kanar

Novagen

 pC-CmACS-badA

pCDFDuet + ACS from Citrus microcarpa + badA from Rhodopseudomonas palustris

This study

 pC-RgACS-badA

pCDFDuet + ACS from Ruta graveolens + badA from R. palustris

This study

 pC-RgACS-pqsA

pCDFDuet + ACS from Ruta graveolens + pqsA from Pseudomonas aeruginosa

This study

 pC-CmACS-pqsA

pCDFDuet + ACS from C. microcarpa + pqsA from P. aeruginosa

This study

 pE-RgACS-badA

pETDuet + ACS from Ruta graveolens + badA from R. palustris

This study

 pE-RgACS-pqsA

pETDuet + ACS from Ruta graveolens + pqsA from P. aeruginosa

This study

 pR-RgACS-badA

pRSFDuet + ACS from Ruta graveolens + badA from R. palustris

This study

 pA-accABCD

pACYCDuet + accABCD from Photorhabdus luminescens

Kim et al. [32]

 pA-Acs

pACYCDuet + Acs from Escherichia coli

This study

 pA-ackA-pta

pACYCDuet + ackA-pta from E. coli

This study

 pA-PDHm

pACYCDuet + PDHm from E. coli

This study

 pC-trpE

pCDFDuet + trpE from E. coli

This study

 pC-aroG-trpE

pCDFDuet + aroG from Escherichia coli in the first multiple cloning site (MCS1) + trpE from E. coli in the second MCS (MCS2)

This study

 pC-aroGf-trpE

pCDFDuet + aroGf from E. coli in MCS1 + trpE in MCS2

This study

 pC-aroGf-ppsA-tktA-trpE

pCDFDuet + aroGf, ppsA and tktA from E. coli in MCS1 + trpE in MCS2

This study

 pC-aroL-aroGf-ppsA-tktA-trpE

pCDFDuet + aroL, aroGf, ppsA and tktA from E. coli in MCS1 + trpE in MCS2

This study

 pC-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-trpE

pCDFDuet + aroL, aroE, aroD, aroB, aroGf, ppsA and tktA from E. coli in MCS1 + trpE in MCS2

This study

 pC-NMT

pCDFDuet + NMT from Ruta graveolens

This study

 pC-NMT-trpE

pCDFDuet + NMT from R. gravealens + trpE from E. coli

This study

 pC-aroG-NMT-trpE

pCDFDuet + aroG from E. coli in MCS1 + NMT and trpE in MCS2

This study

 pC-aroGf-NMT-trpE

pCDFDuet + aroGf from E. coli in MCS1 + NMT and trpE in MCS2

This study

Strains

  

 DH5α

F– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK–, mK +) phoA supE44 λ− thi-1 gyrA96 relA1

Novagen

 BL21 (DE3)

FompT hsdSB(rB mB) gal dcm lon (DE3)

Novagen

 B-DHA1

E. coli BL21 (DE3) harboring pC-RgACS-BadA

This study

 B-DHA2

E. coli BL21 (DE3) harboring pC-CmACS-BadA

This study

 B-DHA3

E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-TrpE

This study

 B-DHA4

E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-aroG-TrpE

This study

 B-DHA5

E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-aroGf-TrpE

This study

 B-DHA6

E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-trpE

This study

 B-DHA7

E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-TrpE

This study

 B-DHA8

E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-aroG-trpE

This study

 B-DHA9

E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-aroGf-trpE

This study

 B-DHA10

E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-trpE

This study

 B-NMA1

E. coli BL21 (DE3) harboring pC-RgACS-PqsA

This study

 B-NMA2

E. coli BL21 (DE3) harboring pC-CmACS-PqsA

This study

 B-NMA3

E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-PlaccABCD

This study

 B-NMA4

E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-ack-pta

This study

 B-NMA5

E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-mPDH

This study

 B-NMA6

E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-acs

This study

 B-NMA7

E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pACYCD

This study

 B-NMA8

E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-aroG-NMT-TrpE, pA-PlaccABCD

This study

 B-NMA9

E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-aorGf-NMT-TrpE, pA-PlaccABCD

This study