Plasmids or E. coli strain | Relevant properties or genetic marker | Source or reference |
---|---|---|
Plasmids | ||
pACYCDuet-1 | P15A ori, Cmr | Novagen |
pCDFDuet-1 | CloDE13 ori, Strr | Novagen |
pETDuet-1 | f1 ori, Ampr | Novagen |
pRSFDuet-1 | RSF ori, Kanar | Novagen |
pC-CmACS-badA | pCDFDuet + ACS from Citrus microcarpa + badA from Rhodopseudomonas palustris | This study |
pC-RgACS-badA | pCDFDuet + ACS from Ruta graveolens + badA from R. palustris | This study |
pC-RgACS-pqsA | pCDFDuet + ACS from Ruta graveolens + pqsA from Pseudomonas aeruginosa | This study |
pC-CmACS-pqsA | pCDFDuet + ACS from C. microcarpa + pqsA from P. aeruginosa | This study |
pE-RgACS-badA | pETDuet + ACS from Ruta graveolens + badA from R. palustris | This study |
pE-RgACS-pqsA | pETDuet + ACS from Ruta graveolens + pqsA from P. aeruginosa | This study |
pR-RgACS-badA | pRSFDuet + ACS from Ruta graveolens + badA from R. palustris | This study |
pA-accABCD | pACYCDuet + accABCD from Photorhabdus luminescens | Kim et al. [32] |
pA-Acs | pACYCDuet + Acs from Escherichia coli | This study |
pA-ackA-pta | pACYCDuet + ackA-pta from E. coli | This study |
pA-PDHm | pACYCDuet + PDHm from E. coli | This study |
pC-trpE | pCDFDuet + trpE from E. coli | This study |
pC-aroG-trpE | pCDFDuet + aroG from Escherichia coli in the first multiple cloning site (MCS1) + trpE from E. coli in the second MCS (MCS2) | This study |
pC-aroGf-trpE | pCDFDuet + aroGf from E. coli in MCS1 + trpE in MCS2 | This study |
pC-aroGf-ppsA-tktA-trpE | pCDFDuet + aroGf, ppsA and tktA from E. coli in MCS1 + trpE in MCS2 | This study |
pC-aroL-aroGf-ppsA-tktA-trpE | pCDFDuet + aroL, aroGf, ppsA and tktA from E. coli in MCS1 + trpE in MCS2 | This study |
pC-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-trpE | pCDFDuet + aroL, aroE, aroD, aroB, aroGf, ppsA and tktA from E. coli in MCS1 + trpE in MCS2 | This study |
pC-NMT | pCDFDuet + NMT from Ruta graveolens | This study |
pC-NMT-trpE | pCDFDuet + NMT from R. gravealens + trpE from E. coli | This study |
pC-aroG-NMT-trpE | pCDFDuet + aroG from E. coli in MCS1 + NMT and trpE in MCS2 | This study |
pC-aroGf-NMT-trpE | pCDFDuet + aroGf from E. coli in MCS1 + NMT and trpE in MCS2 | This study |
Strains | ||
DH5α | F– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK–, mK +) phoA supE44 λ− thi-1 gyrA96 relA1 | Novagen |
BL21 (DE3) | F−ompT hsdSB(r−B m−B) gal dcm lon (DE3) | Novagen |
B-DHA1 | E. coli BL21 (DE3) harboring pC-RgACS-BadA | This study |
B-DHA2 | E. coli BL21 (DE3) harboring pC-CmACS-BadA | This study |
B-DHA3 | E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-TrpE | This study |
B-DHA4 | E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-aroG-TrpE | This study |
B-DHA5 | E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-aroGf-TrpE | This study |
B-DHA6 | E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-trpE | This study |
B-DHA7 | E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-TrpE | This study |
B-DHA8 | E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-aroG-trpE | This study |
B-DHA9 | E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-aroGf-trpE | This study |
B-DHA10 | E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-trpE | This study |
B-NMA1 | E. coli BL21 (DE3) harboring pC-RgACS-PqsA | This study |
B-NMA2 | E. coli BL21 (DE3) harboring pC-CmACS-PqsA | This study |
B-NMA3 | E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-PlaccABCD | This study |
B-NMA4 | E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-ack-pta | This study |
B-NMA5 | E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-mPDH | This study |
B-NMA6 | E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-acs | This study |
B-NMA7 | E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pACYCD | This study |
B-NMA8 | E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-aroG-NMT-TrpE, pA-PlaccABCD | This study |
B-NMA9 | E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-aorGf-NMT-TrpE, pA-PlaccABCD | This study |