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Table 1 Plasmids and strains used in the present study

From: Synthesis of acridone derivatives via heterologous expression of a plant type III polyketide synthase in Escherichia coli

Plasmids or E. coli strainRelevant properties or genetic markerSource or reference
Plasmids
 pACYCDuet-1P15A ori, CmrNovagen
 pCDFDuet-1CloDE13 ori, StrrNovagen
 pETDuet-1f1 ori, AmprNovagen
 pRSFDuet-1RSF ori, KanarNovagen
 pC-CmACS-badApCDFDuet + ACS from Citrus microcarpa + badA from Rhodopseudomonas palustrisThis study
 pC-RgACS-badApCDFDuet + ACS from Ruta graveolens + badA from R. palustrisThis study
 pC-RgACS-pqsApCDFDuet + ACS from Ruta graveolens + pqsA from Pseudomonas aeruginosaThis study
 pC-CmACS-pqsApCDFDuet + ACS from C. microcarpa + pqsA from P. aeruginosaThis study
 pE-RgACS-badApETDuet + ACS from Ruta graveolens + badA from R. palustrisThis study
 pE-RgACS-pqsApETDuet + ACS from Ruta graveolens + pqsA from P. aeruginosaThis study
 pR-RgACS-badApRSFDuet + ACS from Ruta graveolens + badA from R. palustrisThis study
 pA-accABCDpACYCDuet + accABCD from Photorhabdus luminescensKim et al. [32]
 pA-AcspACYCDuet + Acs from Escherichia coliThis study
 pA-ackA-ptapACYCDuet + ackA-pta from E. coliThis study
 pA-PDHmpACYCDuet + PDHm from E. coliThis study
 pC-trpEpCDFDuet + trpE from E. coliThis study
 pC-aroG-trpEpCDFDuet + aroG from Escherichia coli in the first multiple cloning site (MCS1) + trpE from E. coli in the second MCS (MCS2)This study
 pC-aroGf-trpEpCDFDuet + aroGf from E. coli in MCS1 + trpE in MCS2This study
 pC-aroGf-ppsA-tktA-trpEpCDFDuet + aroGf, ppsA and tktA from E. coli in MCS1 + trpE in MCS2This study
 pC-aroL-aroGf-ppsA-tktA-trpEpCDFDuet + aroL, aroGf, ppsA and tktA from E. coli in MCS1 + trpE in MCS2This study
 pC-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-trpEpCDFDuet + aroL, aroE, aroD, aroB, aroGf, ppsA and tktA from E. coli in MCS1 + trpE in MCS2This study
 pC-NMTpCDFDuet + NMT from Ruta graveolensThis study
 pC-NMT-trpEpCDFDuet + NMT from R. gravealens + trpE from E. coliThis study
 pC-aroG-NMT-trpEpCDFDuet + aroG from E. coli in MCS1 + NMT and trpE in MCS2This study
 pC-aroGf-NMT-trpEpCDFDuet + aroGf from E. coli in MCS1 + NMT and trpE in MCS2This study
Strains  
 DH5αF– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK–, mK +) phoA supE44 λ− thi-1 gyrA96 relA1Novagen
 BL21 (DE3)FompT hsdSB(rB mB) gal dcm lon (DE3)Novagen
 B-DHA1E. coli BL21 (DE3) harboring pC-RgACS-BadAThis study
 B-DHA2E. coli BL21 (DE3) harboring pC-CmACS-BadAThis study
 B-DHA3E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-TrpEThis study
 B-DHA4E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-aroG-TrpEThis study
 B-DHA5E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-aroGf-TrpEThis study
 B-DHA6E. coli BL21 (DE3) harboring pE-RgACS-BadA, pA-PlaccABCD, pC-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-trpEThis study
 B-DHA7E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-TrpEThis study
 B-DHA8E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-aroG-trpEThis study
 B-DHA9E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-aroGf-trpEThis study
 B-DHA10E. coli BL21 (DE3) harboring pR-RgACS-BadA, pA-PlaccABCD, pC-aroL-aroE-aroD-aroB-aroGf-ppsA-tktA-trpEThis study
 B-NMA1E. coli BL21 (DE3) harboring pC-RgACS-PqsAThis study
 B-NMA2E. coli BL21 (DE3) harboring pC-CmACS-PqsAThis study
 B-NMA3E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-PlaccABCDThis study
 B-NMA4E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-ack-ptaThis study
 B-NMA5E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-mPDHThis study
 B-NMA6E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pA-acsThis study
 B-NMA7E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-NMT-TrpE, pACYCDThis study
 B-NMA8E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-aroG-NMT-TrpE, pA-PlaccABCDThis study
 B-NMA9E. coli BL21 (DE3) harboring pE-RgACS-PqsA, pC-aorGf-NMT-TrpE, pA-PlaccABCDThis study